A cryotechnique-based method for low abundance protein immunolocalization in tomato (Solanum lycopersicum) roots infected with a nematode, Globodera rostochiensis
نویسندگان
چکیده
منابع مشابه
Reproduction of Globodera rostochiensis on Transformed Roots of Solanum tuberosum cv. Desiree.
Transformed roots of the susceptible potato Solanum tuberosum L. cv. Desiree were inoculated with second-stage juveniles (J2) of Globodera rostochiensis pathotype Rol. Adult males emerged after 3-4 weeks and matings with females occurred. After 8 weeks gentle pressure on the eggs of maturing females released the J2 which were viable. Because this technique enables the production of vigorously g...
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(1)H nuclear magnetic resonance (NMR)-based metabolomics has been applied to study the compatible interaction between tomato plants and Tomato Mosaic Virus (ToMV). A detailed time course of metabolic fingerprinting of ToMV-inoculated and non-inoculated systemically infected tomato leaves has provided a fundamental understanding of the metabolic state of the plant not only in response to ToMV in...
متن کاملMajor Sperm Protein Genes from Globodera rostochiensis.
Three genes in the major sperm protein (MSP) gene family from the potato cyst nematode Globodera rostochiensis were cloned and sequenced. In contrast to the absence of introns in Caenorhabditis elegans MSP genes, these genes in G. rostochiensis contained a 57 nucleotide intron, with normal exon-intron boundaries, in the same relative location as the intron in Onchocerca volvulus. The MSP genes ...
متن کاملThe effects of Nematode Infection and MI-Mediated Resistance in Tomato (Solanum Lycopersicum) on Plant Fitness
The Mi gene in tomato (Solanum lycopersicum) is a single, dominant resistance (R) gene that confers resistance against several species of insects and root-knot nematodes. This study examined the impact of root-knot nematode infestation and the plant growth and reproduction of near-isogenic tomato cultivars with and without Mi. The objectives of this experiment were to examine the potential fitn...
متن کاملWorm castings-based growing media with biochar and arbuscular mycorrhizal fungi for producing organic tomato (Solanum lycopersicum L.) in greenhouse.
Organic vegetable production has specific research and innovation requirements which are not shared by other parts of the food and farming sector. A pot experiment was conducted to investigate the interactive effects of few permitted organic inputs such as arbuscular mycorrhizal fungi, biochar, and different ratios of peat:worm casting on tomato (Solanum lycopersicum L.) growth, mycorrhizal dep...
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ژورنال
عنوان ژورنال: Micron
سال: 2018
ISSN: 0968-4328
DOI: 10.1016/j.micron.2018.02.003